PURPOSE: Evidence have shown active transportating decreases obesity rates, however separate analysis using either walking or cycling could strengthen this results in adolescents. This study aimed to examine the associations between walking and cycling for active transportation and obesity indicators in Latin American adolescents. METHODS: Population-based study with 671 adolescents (15-17 years) from eight countries participated on the Latin American Study of Nutrition and Health (Argentina, Brazil, Chile, Colombia, Costa Rica, Ecuador, Peru, and Venezuela). Walking and cycling for active transportation were measured using the International Physical Activity Questionnaire long version. Body mass index (Kg/m2), waist circumference (cm), neck circumference (cm), and relative fat mass (%) were included as obesity indicators. Linear regression models were estimated on pooled data adjusted for sex, age, socio-economic levels, ethnicity, and leisure-time physical activity. RESULTS: Mean walking and cycling were 22.6 (SD: 33.1) and 5.1 (SD: 24.1) min/week. The median values were 12.8 (IQR: 4.2; 25.7) and 0 (IQR: 0; 6.2) for walking and cycling. Participants reporting ≥10 min/week of walking and cycling for active transportation were 84.2% and 15.5%, respectively. Costa Rica (94.3% and 28.6%) showed the highest time spent on walking and cycling, respectively, while Venezuela (68.3% and 2.4%) showed the lowest. In the overall ELANS sample, there was no significant associations between walking and obesity indicators. Alternatively, cycling was associated with lower body mass index (β: -0.101; 95%CI: -0.143; -0.059), and waist circumference (β: -0.029; 95%CI: -0.038; -0.020), independently of sex, age, socio-economic levels, ethnicity, and leisure-time physical activity. There was no significant association between cycling for active transportation and neck circumference, and relative fat mass. CONCLUSIONS: Cycling for active transportation was negatively associated with obesity indicators, specially body mass index and waist circumference, independently of leisure-time physical activity. Programs for promoting cycling for active transportation should be a feasible strategy to tackle the high obesity rate in adolescents from Latin America.