This study aimed to give guidelines for establishing a psychrophilic biomethane potential (BMP) test by three approaches for inoculum acclimation under 25°C. The first test was run using stabilized mesophilic and stabilized psychrophilic inoculums to digest cellulose. Second assay was based on temperature acclimatization of inoculum just by time. The third approach was running a consecutive BMP test under psychrophilic temperatures. Cellulose psychrophilic BMP using stabilized psychrophilic inoculum achieve similar results to those obtained at mesophilic conditions with stabilized mesophilic inoculum. Time acclimatization of inoculum improve BMP results respect non-adapted (from 0.47 to 055 Nm3CH4 kg-1SV at 20 ºC, from 0.31 to 0.44 Nm3CH4 kg-1SV at 10 ºC). Consecutive BMP under psychrophilic conditions shows better results for inoculum acclimatization to run assays at low temperatures: from 0.20 to 0.37 Nm3CH4 kg-1SV at 23 ºC. Accordingly, there is a need to develop and standardize a procedure to run psychrophilic BMP.