A new species of the genus Tenedos O. Pickard-Cambridge, 1897 belonging to the barronus group is described and illustrated based on both sexes. Tenedos barbarae sp. n. is described from Serranía del Perijá in Cesar department, Colombia. Specimens of the new species were collected during a standardized sampling in a landscape of tropical dry forest. Notes on ecology and natural history are provided. Additionally, a map on the spatial disposition of the new species in the landscape is included.
