Cundinamarca is the major potato producer department of Colombia. Potato growers in Cundinamarca reported the presence of a new set of symptoms affecting commercial potato fields as far back as 2012. The initial objective of this work was to test the association of the reported symptoms with phytoplasmas. Phytoplasmas were detected by nested PCR of the 16S rRNA gene and ‘ Candidatus Phytoplasma asteris’ (group 16SrI) and ‘ Candidatus Phytoplasma fraxini’ (16SrVII) were found by RFLP and sequencing analysis in plants from commercial plots. Of the 152 symptomatic and non-symptomatic potato plants tested, 33,5% were positive for phytoplasmas in infections with either one phytoplasma or both phytoplasmas (mixed infections). Phytoplasmas were also detected by electron microscopy in symptomatic plants. A study was conducted to test if the phytoplasmas were transmitted from infected plants to daughter plants via tubers, as was the case for both species of phytoplasmas. We demonstrated that the insect vector Exitianus atratus (Hemiptera: Cicadellidae) transmits both ‘ Ca. P. asteris’ and ‘ Ca. P. fraxini’ to potato plants, and that the infection generated the same symptoms observed in field infected plants. Phytoplasmas of either species in single or mixed infections, produced indistinguishable symptoms in the infected plants. In conclusion, symptoms including foliage yellowing or discoloration, curly leaves to the upper leaf side, leaves with purple margins, and less commonly purple coloration of the underside veins or of the leaf lamina, distortions of the normal shape of the plant due to excessive shoot proliferation, abnormally short or long internodes, or abnormal morphology of the leaves, were associated to ‘ Ca. P. asteris’ and ‘ Ca. P. fraxini’ in Cundinamarca.