Mineral nutrition in arracacha is a critical production factor that conditions harvest yield. Few research works have been developed in nutrition and physiology areas, which do not allow the design of ideal fertilization programs; consequently, they have increased production costs, soil degradation, and low-quality storage roots. Therefore, this study aimed to characterize the symptoms associated with macronutrient deficiency in arracacha plants and its effect on morphological parameters, the accumulation of fresh and dry biomass, and the distribution of dry matter in the different organs. Under greenhouse conditions, the experiment was conducted in Cajamarca, Tolima, Colombia. A completely randomized design was implemented, with seven treatments and six replicates (6 solutions lacking N, P, K, Ca, Mg, and S and Hoagland complete solution). Forty-two seedlings were transplanted to which the complete solution was applied for 75 days, increasing the concentrations from 0.25 M to 0.5 M and 1 M, and then nutritional deficiencies were induced. Deficiencies caused by macronutrients in A. xanthorrhiza plants exhibited visual symptoms and changes in their morphology. The omission of N, Ca, S, P, Mg, and K reduced plant height, leaf width, leaf number, and fresh and dry mass of leaves, petioles, storage roots, and propagules. Deficient plants allocated more significant dry mass accumulation to strain, storage roots, propagules, and leaves.