A great variety of microorganisms need dipterans as part of their life cycle. The dominance of these insects as vectors is differential between vertebrates; thus, they have a leading role in transmitting viruses, protozoans, and other pathogens to mammals and birds; however, they have a more discrete representation as vectors of parasites among ectotherms. Most of the parasites that affect herpetofauna are transmitted mainly by mites, ticks, leeches. At the same time, in less proportion use generalist or herpetophilic dipterans, which have developed strategies to extend their feeding sources to warm-blooded vertebrates. On the other hand, hemoparasites belonging to Apicomplexa, Trypanosomatida, and Spirurida have generated mechanisms for dissemination other than the infection by bite. This chapter aims to provide a detailed review of the current knowledge of the hemoparasites of herpetofauna transmitted by dipterans, collecting classic literature until recent research. We also discuss the life cycle transmission of these parasites and their possible implications in public health scenarios.