Antibacterial and antioxidant effects of alcoholic extract for the Cucurbita pepo plant were studied. Properties optical (UV-visible spectrophotometer ) and morphological surface(scanning electron microscopy ) detection of the alcoholic extract was done, and the antibacterial activity was investigated against pathogenic microbes (E.coli, Staphylococcus aureus). Results showed the highest effect for crud extract in the growth of E.coli by the zone of inhibition diameter reached (16.33±0.58 ), followed by Staph. Aureus by the diameter of the inhibition zone reached (12.33±2.30mm). The results indicate that the plant C. pepo can be considered a valuable source of effective antioxidant agents at a 0.8 µg/ml concentration. Keywords. Cucurbita pepo plant: Alcoholic extract: Antioxidant: Antibacterial Activity