Presents the list of fish fauna gathered during a scientific expedition to Cayo Albuquerque (12°07’N, 81°52’2” W) Colombia, the southernmost cay of the Archipelago of San Andrés, Providencia and Santa Catalina, which includes the Seaflower Biosphere Reserve. Data was collected through visual censuses in coral reef formations and seagrass beds, between September 23 and October 3, 2018. A total of 191 species were registered, distributed in 50 families, with Labridae (24) and Serranidae (22) showing the highest species richness. Twenty-five species are new reports for Albuquerque.