Event Abstract Back to Event New Strategy in Spinal Cord Repair with Cryofrozen Primary Cell Cultures in Model In Vivo Rosa M. Gomez Bello1*, Magdy Y. Sanchez Molina1, 2, Daniela Vargas Brochero1, 3 and Lucia Botero Espinosa4 1 Fundación en Neuroregeneracion, Colombia 2 Nacional de Colombia, Facultad de Medicina, Colombia 3 De La Sabana, Facultad de Medicina, Colombia 4 Nacional de Colombia, Facultad de Medicina Veterinaria y Zootecnia, Colombia The approach to knowledge of the biological and functional characteristics of the OECs has prompted the development of studies where these cells have been implemented as a possible therapeutic strategy for injuries of the CNS. Furthermore, the production of different molecules favorable for axonal regeneration and the apparent ease of which the OECs penetrate the CNS, could be important characteristics in favor of the use of OECs. The purpose of the present study was to test the hypothesis that the transplant of cryopreserved OCEs plus aFGF + FG placed in the injured rat ́s, after a surgical trauma, could be capable to produce a regenerative response and to recuperate the motive function of the hind limbs. The investigation was approved by the Ethics Committee of the University of La Sabana. The OCEs cultures were obtained and purified from neonatal rats (10 days) and adult rats (5 months) from which allograft transplants (PNS) were extracted, nerve and external layer Viability  of olfactory bulb (CNS). The cultures were supplemented with DMEM and maintained at 37oC , 98% RH and 5% CO2. This process obtained an average of 8 x 105 cells and reaching a confluence of aproximately 80%. Subsequently, 2 x 105 cells were frozen at minus 192 oC (DMEM + FBS at 20% in DMSO at 10%). After approximately eight months we proceed to thaw cryogenic viales with the OECs 1.5 x 106 were used, injected in the center of the medullar cavity of the severe injury of the spinal cord of the experimental model. The rats that were subjected to surgery were maintained under observation for 42 days. The comparative study of treatments showed differences that are statistically significative with respect to the experimental group with the OECs cryopreserved + aFGF + FG reaching twelve points in the evaluation with the BBB scale. The results as show evidence that when evaluating the locomotor recuperation, after a medullar section in rats through the implantation of OECs of cryopreserved cells combined with aFGF + FG. In addition, the conclusion it is potencial use in reparative phenomena in the injured medullar tissue but making further studies necessary before considering their use. Figure 1 Acknowledgements Research was sponsored by generous financial support of patrimonial fund of Universidad de la Sabana. Bogota-Colombia Colciencias Grant 377-09 References Claire Terry, Anil Dhawan, Ragai R. Mitry, Robin D. Hughes.(2006). Cryopreservation of isolated human hepatocytes for transplantation: State of the art. Cryobiology 53 pg.149–159. Guangpeng Liu a,*, Heng Zhou a, Yulin Li a, Gang Li a, Lei Cui a,b,*, Wei Liu a,b, Yilin Cao a,b. (2008). Evaluation of the viability and osteogenic differentiation of cryopreserved human adipose-derived stem cells. Cryobiology 57 pg. 18–24 Bingkun K. Chen a, Andrew M. Knight a, Nicolas N. Madigan a, LouAnn Gross a, Mahrokh Dadsetan b, Jarred J. Nesbitt a, Gemma E. Rooney a, Bradford L. Currier b, Michael J. Yaszemski b, Robert J. Spinner c, Anthony J. Windebanka. (2011). Comparison of polymer scaffolds in rat spinal cord: A step toward quantitative assessment of combinatorial approaches to spinal cord repair. Biomaterials 32 pg. 8077- 8086. Raffaella Adami, Giuseppe Scesa, and Daniele Bottai*.(2014). Stem cell transplantation in neurological diseases: improving effectiveness in animal models. Front Cell Dev Biol; 2: 17 Keywords: Spinal Cord Injuries, Cryobiology, Olfactory Mucosa, Cells, Cultured, Animal Models Conference: Latin-American School on glial cells in the diseased brain (IBRO), Bogotá, Colombia, 13 Jul - 17 Jul, 2015. Presentation Type: Oral Presentation Topic: Glial response to spinal cord injury Citation: Gomez Bello RM, Sanchez Molina MY, Vargas Brochero D and Botero Espinosa L (2015). New Strategy in Spinal Cord Repair with Cryofrozen Primary Cell Cultures in Model In Vivo. Conference Abstract: Latin-American School on glial cells in the diseased brain (IBRO). doi: 10.3389/conf.fncel.2015.35.00020 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Feb 2015; Published Online: 11 Jun 2015. * Correspondence: Dr. Rosa M Gomez Bello, Fundación en Neuroregeneracion, Bogota, Cundinamarca, 0057, Colombia, rmgomez@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rosa M Gomez Bello Magdy Y Sanchez Molina Daniela Vargas Brochero Lucia Botero Espinosa Google Rosa M Gomez Bello Magdy Y Sanchez Molina Daniela Vargas Brochero Lucia Botero Espinosa Google Scholar Rosa M Gomez Bello Magdy Y Sanchez Molina Daniela Vargas Brochero Lucia Botero Espinosa PubMed Rosa M Gomez Bello Magdy Y Sanchez Molina Daniela Vargas Brochero Lucia Botero Espinosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
