Event Abstract Back to Event Analysis of HLA class II alleles (DQ - DR) in patients with gastric diseases caused by Helicobacter pylori, in the city of Barranquilla, Colombia. Franklin E. Torres1*, Aracely García1* and Marena Rodriguez1* 1 Universidad Libre Seccional Barranquilla, Atlántico, Colombia Introduction Stomach disorders represent one of the most common disorders of the digestive tract, represented by a variety of clinically relevant phenotypes. In words: acute gastritis, acute gastric ulcer, chronic gastritis, autoimmune gastritis, peptic ulcer disease, mucosal atrophy, intestinal metaplasia and dysplasia, cystic gastritis, gastric adenocarcinoma among others (1). In Colombia, gastric cancer is the leading cause of cancer deaths, figures are on the rise and truly represent a public health problem at the national level (2). Regarding the genesis of gastropathies have been involved several vital factors such as: environmental factors, genetic factors and psychosocial environment or context; making a synergist triad facilitates the initiation and progress of lesions in the gastric mucosa (3). Among the environmental factors we can mention Helicobacter pylori infection and uncontrolled consumption of NSAIDs, as the most studied worldwide (4,5), while at the genetic level are the molecules of the major histocompatibility complex, especially those HLA class II (DR and DQ), and some polymorphisms in the Toll Like Receptor (TLR) and some proinflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF α) (6-8). H. pylori infection is the most common worldwide bacterial infection (14). In 1994, the International Agency for Research on Cancer (IARC: International Agency for Research on Cancer) cataloged H. pylori as a type I carcinogen (15). H. pylori infection increases the risk of gastric cancer up to 6 times more than individuals without the infection. Colombia has a prevalence of H. pylori infection of 86% in adults over 20 years (16) and 80% in children older than 8 years (17). Persistent infection of the gastric mucosa can generate inflammatory response, silent processes or, in the worst cases can progress to gastric adenocarcinoma (18,19). In this research we immunogenetic aspects related to the distribution of HLA Class II (DR and DQ) in a group of patients with gastric pathologies associated with H. pylori and a control group of the city of Barranquilla, Colombia. Materials and methods A study of cases and controls, where the distribution of HLA-DQ and HLA-DR in the two groups were analyzed was performed. A total of 98 participants, of which 45 were patients with gastric diseases associated with H. pylori (confirmed by biopsy and histopathology) and 53 belonged to the control group, which showed no symptoms of dyspepsia or another manifestation of gastropathy in particular. All participants were over 18 years. All they read and signed the informed prior research linking the consent. - Collection and purification of genomic DNA: Genomic DNA was extracted from a sample of venous blood using Wizard Genomic Kit (Promega ™, USA) following the manufacturer's instructions (20). - Terms of amplification (PCR) of HLA-DQ and HLA-DR: The amplification reaction was carried out in the laboratory of Molecular Biology at the Free University Sectional Barranquilla. Using the thermal cycler PTC-100 MJ Research, following the reaction conditions of the PCR-SSP (polymerase chain reaction-sequence specific priming) technique (21). - Genotyping of HLA DQ and DR: Genotyping was performed using the "DNA Micro SSP ™ Generic HLA Class II DNA Typing Tray One Lambda Inc" method (21). - Electrophoresis of amplification products: The amplicons were run on agarose gel 2.5% TBE1X buffer. Electrophoresis was performed at 145 volts until the guide dye has migrated about 0.5 cm into the gel (about 3-5 minutes or so). - Statistical analysis: For qualitative variables, the frequency data were summarized as percentages. Statistically significant differences between groups were tested by Pearson Chi-square (χ2), with Yates correction. The risk was estimated by calculating the odds ratio (OR) with a confidence interval of 95% (95%). The P value was determined by Fisher's exact test. A P <0.05 was considered statistically significant. Data were analyzed using SPSS Statistics 20 program. Results and discussion: The case group between 18 and 62 years of age, with a total of 26 women and 19 men. All controls were over 18 and had a distribution of 25 men and 28 women. Gastric pathologies were found in the case group were: chronic gastritis (97.8%) and gastric adenocarcinoma (2.2%). Hiatal hernia (2.2%), ulcerative colitis (2.2%) and chronic Duodenitis (2.2%): extra-gastric diseases such wreaked. Genotyping and distribution of HLA-DQ and HLA-DR was performed by analyzing the exon 2 of the β chain of HLA-DQB1, HLA-DRB1, HLA-DRB3, HLA-DRB4 and HLA-DRB5 genes. The findings in our investigation established a strong association between chronic gastritis with HLA-DRB1 * 0701, HLA-DRB1 * 0401 and HLA-DRB1 * 1501 allele (p ≤ 0.05, with an OR = ∞). Therefore these alleles contribute to a significant risk in contracting gastritis in Barranquilla population. In the literature search we found that alleles, mentioned above, were associated with a variety of autoimmune diseases. The HLA-DRB1 * 0701 molecule is associated with: vulgar Psoriasis and Crohn's disease in Taiwan (RR = 6.4, corrected P-value, Pc 0.05 (p = 0.179). This statistically significant association may be due to insufficient sample size or the analyzed population it is not in Hardy-Weinberg equilibrium for this locus (HLA-DRB1). Acknowledgements Our work has been funded by the Research Centre of the Free University (CIUL) Barranquilla branch. References 1. Lee EL, et al. Gastritis and gastropathies. In: Feldman M, et al. Sleisenger & Fordtran's Gastrointestinal and Liver Disease: Pathophysiology, Diagnosis, Management. 9th ed. Philadelphia, Pa.: Saunders Elsevier; 2010.Disponible en: http://www.mdconsult.com/books/about.do?eid=4-u1.0-B978-1-4160-6189. Acceso June 9, 2013. 2. International Agency for Research on Cancer. Globocan 2012. Fecha de consulta: 12 de junio de 2014. 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Sakai T, Aoyama N, Satonaka K, Shigeta S, Yoshida H, Shinoda Y, Shirasaka D, Miyamoto M, Nose Y, Kasuga M. HLA-DQB1 locus and the development of atrophic gastritis with Helicobacter pylori infection. J Gastroenterol. 1999;34(Suppl 11):24-27). 35. Yoshinori Watanabe, Nobuo Aoyama, Toshiyuki Sakai, Daisuke Shirasaka, Shuji Maekawa, Kohei Kuroda, Casmir Wambura, Takao Tamura, Yoshisuke Nose and Masato Kasuga. HLA-DQB1 locus and gastric cancer in Helicobacter pylori infection. Journal of Gastroenterology and Hepatology 2006;21:420–424. Keywords: Infection, HLA, Helicobacter pylori, Chronic gastritis, Immunity Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Oral Presentation Topic: Infectious and parasitic diseases Citation: Torres FE, García A and Rodriguez M (2015). Analysis of HLA class II alleles (DQ - DR) in patients with gastric diseases caused by Helicobacter pylori, in the city of Barranquilla, Colombia.. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00307 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. 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Received: 13 May 2015; Published Online: 15 Sep 2015. * Correspondence: Prof. Franklin E Torres, Universidad Libre Seccional Barranquilla, Atlántico, Puerto Colombia, Puerto Colombia, +057, Colombia, franklintj654@hotmail.com Prof. Aracely García, Universidad Libre Seccional Barranquilla, Atlántico, Puerto Colombia, Puerto Colombia, +057, Colombia, agarcia@unilibrebaq.edu.co Prof. Marena Rodriguez, Universidad Libre Seccional Barranquilla, Atlántico, Puerto Colombia, Puerto Colombia, +057, Colombia, mrodriguez@unilibrebaq.edu.co Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. 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