The International Cocoa Organization recognized Colombian cacao as "fine aroma" but in recent years clone CCN 51 has grown in hectareas widely due to its high yield. The Tolima department is the fourth producer of cacao in Colombia, but there is a lack of knowledge of the physicochemical properties of locally grown cacao. According to its chemical composition, it is possible to find new markets for differentiated cacao. The objective of this article was to carry out a chemical characterization of four regional materials (UTLP02, UTVE01, UTGC01 and UTLM02) compared to CCN 51. A morphological and physicochemical evaluation profile was performed based on qualitative and quantitative descriptors. Likewise, the total polyphenol content and antioxidant activity were determined in cacao beans previously defatted. Finally, the quantification of theobromine (TB) and caffeine (CF) was performed by HPLC. The TB/CF ratio values classified UTVE01 as Criollo, UTGC01 and UTLM02 as Trinitarian and UTLP02 as Forastero. Although CCN51 is considered a remarkable material in terms of productivity, the UTVE01 native material presented good morphological, chemical and sensorial characteristics making it a promising material for new plantings.