The interest in the knowledge of the oviduct environment is based on the place where fertilization and early embryo development take place. As a part of the reproductive tract, the oviduct also responds to stimuli from ovarian hormones (E2 and P4), whose levels vary depending on estrous phase cycle. The objective of this study is to elucidate the effect of estrous cycle phases (follicular and early and late luteal) on gene expression patterns of Bovine Oviduct Epithelial Cells (BOECs). To achieve this objective, oviducts from slaughterhouse animals were recovered, corresponding to an ipsilateral to ovaries with presence of dominant follicles or corpus luteum (Early - Late luteal phase). BOECs from the isthmus and the ampulla were recovered, and expression patterns of genes related to cytokinesis and mitosis mechanisms ( ROCK , CCN2 ), growth factors ( IGFBP3, EGFR, VEGFA, EGFR ), antioxidants mechanisms ( GPX4 ), apoptosis ( BCL2 ), complement component ( C3 ) energetic metabolism ( AKRIB1 , SLC2A1 ), hormones receptors as ESR and LHCGR and specific glycoproteins ( OVGP ) were analyzed. As a result, an effect of late luteal phase (higher P4 levels) on expression of important antioxidant mechanisms GPX4 , energetic metabolism AKRIB1 , growth factors IGBP3, EGFR and regulators CCN2 related genes in the ampulla are described. Low P4 levels (early luteal phase) influence AKR1B1, IGBP3 and CCN2 , expression. In addition, OVPGP expression may be affected by estrogen in cattle oviduct.