The bioenergy (especially biofuels) production and usehas been worldly encouraged as a possible solution for theenergy crisis caused by fossil fuel extraction and burnt.However, some intrinsic features of the biofuels supplychain generate notable uncertainties. The land use changecan be considered a key issue of the feedstock productionstage. In this work, the carbon replacement time wascalculated for the cases of the oil palm growing in soils forforest, degraded lands and marginal lands. Most cultivatedlands are required to increase the biodiesel production inColombia. Additionally, the greenhouse gas emission fromthe land use change was calculated, when the palm cropsare extended to achieve B20 blends. The results indicatedthat the palm crop growing on degraded and marginallands, as well as palm cultivated in Eastern zone, do notgenerate carbon debt. Moreover, oil palm growing underthese conditions helps to increase the carbon captures ofthese lands. The Eastern zone shows the highest carboncapture power.