This study aimed at assessing the usefulness of antibody reactivity in the diagnosis of pulmonary tuberculosis when evaluating the B-cell responses to the ESAT-6 and Ag85A synthetic peptides, the recombinant antigen and antigen 85 (rAg85) and the non-recombinant PPD antigen.A total of 60 creole individuals were included in this study.Patient group was conformed of 20 individuals, whilst control group was composed by 40 healthy individuals.The B-cell responses of pulmonary tuberculosis patients and healthy individuals were evaluated by an IgG-ELISA.The diagnostic accuracy of these methods was assessed by using receiver operating characteristic (ROC) curve analysis.The results showed that the method using the ESAT-6 peptides, rESAT-6 and PPD antigens revealed low specificities, although these remained limited between 40.0 % and 77.5 %.2; whilst the two remaining methods, anti-12033 IgG and anti-12034 IgG, showed the highest sensitivities of 100.0 % (Negative Predictive Value (NPV)=100.0).Regarding the Ag85A peptides, anti-10998 IgG was found to be the most specific (100.0%, Positive Predictive Value (PPV) =100.0).Whilst anti-11006 IgG showed the highest sensitivity of 95.0 % (NPV=90.0)but was the lowest in specificity (22.5 %, PPV=38.0).Conclusion, the use of highly sensitive and specific peptides may obviate the need for a M tuberculosis culture in the initial diagnostic approach to pulmonary tuberculosis.