Conclusion: Significant resistance variations among SA and several classes of antimicrobial agents were observed in LA countries.Daptomycin showed consistent potency against recent clinical isolates of SA collected in LA medical centers, including MDR strains.Resistance to other compounds did not adversely influence daptomycin potency and with S rates at over 99.9% during the past five years suggests that daptomycin has maintained in vitro activity in LA countries.