Abstract Purpose: To describe the peculiarities in imaging acquisition of fourteen patients with choroidal nevus using the Broad Line Fundus Imaging (BLFI) technology. Study Design: Single-center, retrospective, cross-sectional analysis. Methods: We retrospectively reviewed the clinical records of 14 patients with choroidal nevus. We analyzed the retinal images by deconstructing them into color channels (Red, Green, and Blue) with broad line fundus imaging technology. The lesions were categorized as detectable or undetectable. Detectability was qualitatively characterized as dark or light following assessment by two experienced ophthalmologists. Results: We have found that choroidal nevus is undetectable in the blue channel (BC) (435 - 500 nm) and the green channel (GC) (500-585 nm). The only visible changes are related to the drusen, which appeared in BC and GC as light focal dots, correlated to the yellowish foci in the true-color image. On the red channel (RC) (585-640 nm), all lesions revealed the same pattern: a well-defined dark spot, with enhanced contrast, allowing the better visualization, measuring, and characterization of the nevus when compared with the other color channels, including the true-color imaging. Conclusion : BLFI application in choroidal nevus might be helpful at presentation, refining the diagnostic reliability, and monitoring, as it allows for better detection of alterations in the lesions. The peculiarities of the choroidal nevus are better assessed when using the RC due to its longer wavelength and deeper penetration in the retina and choroid.