Objective: To analyze the investigative actions carried out in universities in relation to people with homosexual sexual orientation and transgender gender identity. Methodology: Systematic review of scientific research, in which 63 were identified for analysis through the application of the PRISMA statement. In this way, a total of 56 articles were obtained that met the required inclusion criteria. Conclusions and discussions: Most of the articles found account for research carried out with the heterosexual population, inquiring about social support, attitudes and construction of meanings towards homosexual sexual orientation and transgender gender identity in the university context. On the other hand, those studies that take the homosexual and transgender population as the main actors, address the process of recognition of sexual orientation and the difficulties to which the community is exposed in the different contexts of interaction.