A new species of Eurhopalothrix Brown & Kempf, E. amati Fiorentino, Tocora and Fernández, n. sp. is described based on workers collected in northwestern Colombia. A taxonomic key and distributional map for the new species in the country are provided. This new species differs from other Eurhopalothrix ants by the configuration of specialized setation, a decrease in its propodeal spines, its irregular metanotum profile, and the triangular shape of the head capsule.
