Abstract A technique is presented to measure the efficiency with which c -jets are mistagged as b -jets (mistagging efficiency) using $$t\bar{t}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mi>t</mml:mi> <mml:mover> <mml:mrow> <mml:mi>t</mml:mi> </mml:mrow> <mml:mrow> <mml:mo>¯</mml:mo> </mml:mrow> </mml:mover> </mml:mrow> </mml:math> events, where one of the W bosons decays into an electron or muon and a neutrino and the other decays into a quark–antiquark pair. The measurement utilises the relatively large and known $$W\rightarrow cs$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mi>W</mml:mi> <mml:mo>→</mml:mo> <mml:mi>c</mml:mi> <mml:mi>s</mml:mi> </mml:mrow> </mml:math> branching ratio, which allows a measurement to be made in an inclusive c -jet sample. The data sample used was collected by the ATLAS detector at $$\sqrt{s} = 13$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:msqrt> <mml:mi>s</mml:mi> </mml:msqrt> <mml:mo>=</mml:mo> <mml:mn>13</mml:mn> </mml:mrow> </mml:math> $$\text {TeV}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mtext>TeV</mml:mtext> </mml:math> and corresponds to an integrated luminosity of 139 fb $$^{-1}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msup> <mml:mrow/> <mml:mrow> <mml:mo>-</mml:mo> <mml:mn>1</mml:mn> </mml:mrow> </mml:msup> </mml:math> . Events are reconstructed using a kinematic likelihood technique which selects the mapping between jets and $$t\bar{t}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mi>t</mml:mi> <mml:mover> <mml:mrow> <mml:mi>t</mml:mi> </mml:mrow> <mml:mrow> <mml:mo>¯</mml:mo> </mml:mrow> </mml:mover> </mml:mrow> </mml:math> decay products that yields the highest likelihood value. The distribution of the b -tagging discriminant for jets from the hadronic W decays in data is compared with that in simulation to extract the mistagging efficiency as a function of jet transverse momentum. The total uncertainties are in the range 3–17%. The measurements generally agree with those in simulation but there are some differences in the region corresponding to the most stringent b -jet tagging requirement.