The aim of this study was to analyze the measurement invariance of a scale of academic emotional exhaustion among university students in Peru (n = 472; 76.06% women; Mage = 20.64), Mexico (n = 362; 66.3% women; Mage = 21.06), and Colombia (n = 288; 88.19% women; Mage = 21.78). The internal structure was analyzed with a confirmatory factor analysis in order to verify the single dimensionality of the Emotional Exhaustion Scale (ESS) in each sample, whereas the model’s invariance was analyzed with a multi-group factor analysis. The results showed that the one-dimensional model shows favorable evidence in each sample regard their factor loadings and reliability, and it was invariant across countries regarding its structure (configural invariance), factor loadings (metric invariance), thresholds (strong invariance), and residuals (strict invariance). Although additional research is needed, the ECE appears to be a promising measure to use among Peruvian, Mexican, and Colombian college students.