This study assessed the morphological and nutritional diversity induced through gamma irradiation in Lehmann’slove grass. Seed were irradiated at doses of 0, 100, 200, 300, 450, 600, 900, and 1400 Gy. Ten agronomic traits relatedwith forage quality were evaluated and used to select the mutants, which were confirmed by cluster analysis andmultivariate analysis of variance and then characterized by nutritional and molecular characterization. Mutants with16–20% less (p<0.05) lignin and 36–68% more protein content than the control genotype were found. Genetic distances of 0.38 and 0.49 also revealed differences (p<0.05) between the mutants and control genotype. The phenotypic and genetic variability, induced through gamma irradiation, resulted in the identification of two first generation mutants with outstanding agronomic traits and nutritional quality.