The main goal of a Life cycle analysis —LCA— (in this particular case) is to study the environmental impacts of current Colombian Biofuels: sugarcane–based ethanol (EtOH), and palm oil-based biodiesel. This involves studying their complete life cycle, and their comparison with reference fossil fuels used in Colombia (regular gasoline and diesel fuel). Furthermore, LCA seeks to identify optimization potential for biofuel production in a more friendly way to the environment. Similar approaches have been considered in the literature and they have provided fruitful results for policy design (Khatiwada, Seabra, Silveira, & Walter, 2012). Finally, this LCA study proffers to gather some data to implement the Sustainability Quick Check for Biofuels tool (SQCB).