The energy transition from fossil fuels to renewable fuels is constantly developing and implementing. It is the subject of worldwide research, seeking to curb climate change and the negative effects of pollution. An economic sector worldwide that generates a large amount of pollutants is the transport sector, which only uses fossil fuels. Accordingly, it is necessary to test renewable alternatives that reduce the pollution caused by the sector, trying to use the current automotive fleet. The purpose of this paper is to show the effects of the direct injection of hydrogen gas obtained using an alkaline electrolysis system in a single-cylinder internal combustion engine operated with Diesel fuel, managing to study the effects of hydrogen injection on the different engine parameters such as fuel consumption, rpm, torque, temperature, and exhaust gas analysis, performing a statistical analysis that allows determining the input variables that most influence the decrease in polluting gases CO and CO2 and the variation in fuel consumption in addition to engine performance.