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Conjugation evaluation of African oil palm biochar derived Carbon Dots with anti -BSA

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Abstract:

The present study synthetized Carbon Dots (CDots) obtained from Elaeis guineensis biochar and then conjugated it with a model molecule, in this case, an antibody of Bovine albumin serum (Anti - BSA). The CDots had a size of 2.5 ± 0.7 nm determined through a High-Resolution Transmission Electronical Microscopy (HR-TEM), a Z potential of -56.1 ± 2.37 mV and a Quantum yield of 1.69%, with emission and excitation wavelengths of 428 and 320 nm, respectively. As a bioreceptor molecule, the anti-BSA conjugated to the CDots surface using the carbodiimide method. With the implementation of this technology, it will contribute to the improvement of the quality of life of Colombian vulnerable populations while contributing to the closing of cycles of the oil palm industry by implementing it in the development of optical biosensors for the detection of different health biomarkers like TB antibodies or pesticides in water.

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Carbon and Quantum Dots Applications

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