Due to the COVID-19 pandemic, food e-commerce and delivery demand increase radically in 2020. Indeed, the Colombian delivery cyclist population growth from twenty thousand in 2019 to approximately fifty thousand in 2020. Some unconsidered factors might have affected the delivery cyclist biomechanical behavior and their health. Also, the growth in this population implies more people are affected if they do not consider biomechanical factors in the sporting gesture. Thus, this work aimed to analyze the loading effect on the delivery cyclist posture and related health issues. Kinetic and kinematic analysis of delivery cyclist posture under several conditions has been performed using computational models and motion capture analysis. The results presented in this work suggest that the cranioventral and shoulder sagittal angular variations are affected by the loading increase. Furthermore, the computational model indicates an accumulation of compressive and tensile stress focused on the lumbar area, indicating the need for a new backpack design. In conclusion, this work presented the dangerous effects of load-carrying in the delivery cyclist posture and proposed new backpack design alternatives to solve this problem.