There are several types of biosensors, among them are optical biosensors, which have been of great interest because they allow a response in real time. Recently, optical biosensors based on carbon dots (CDs) have been developed and an increase in sensitivity and specificity has been evidenced due to CDs are a material that has fluorescent properties much better than those of traditional fluorescent markers.Taking into account the aforementioned, in this study the fluorescence phenomenon of CDs conjugated with bovine serum albumin (BSA) was evaluated through the quenching of fluorescence with graphene oxide (GO) and the recovery of fluorescence with BSA antibody (anti-BSA) as a starting point for their potential application as optical biosensor in the research line of 'Tecnologías para la detección de biomarcadores' from EIA University. First of all the CDs were characterized by FTIR, DLS, ζ potential and fluorescence spectroscopy, then the CDs were conjugated with BSA (CDs-BSA) by adsorption and by the carbodiimide conjugation method, and at last CD-BSA fluorescence was quenched with GO (CDs-BSA-GO) and recovered with anti-BSA. The results obtained showed that GO (about 25 ppm) can quencher CDs-BSA (about 125 ppm) fluorescence by 45 %, in addition, it was found that anti-BSA (0.5 μg/mL) can recover CDs-BSA-GO fluorescence by 22 %.