Two distinctive new species of the Neotropical stingless bee genus Paratrigona Schwarz from Ecuador and Paraguay are described and figured. The Ecuadorian species, P. scapisetosa sp. n., belongs to the haeckeli-lineatifrons group and is easily distinguished from its congeners by the unique shape and pubescence of the antennal scape, which is distinctly convex on its outer margin and bears thick, long, simple hairs along its inner margin. The Paraguayan species, P. wasbaueri sp. n., belongs to the lineata group and is easily distinguished by the pattern of body pubescence in both sexes and male genitalic characters. The male of the Mesoamerican species P. ornaticeps (Schwarz) is described and figured. New geographical records for P. impunctata and P. opaca, and an updated key to the haeckeli-lineatifrons and lineata species groups are provided.