Abstract In this work, three lignocellulosic residues, coconut fiber, sugarcane bagasse, and fique bagasse, were used as a carbon source for fungal colonization had been proposed to form a composite material. The lignocellulosic residues presented a high percentage of carbon. The lignocellulosic residues had been conditioned and characterized morphologically. The particle sizes chosen were 16 and 50 sieves. The mycelial growth kinetics of the fungi had been evaluated in the residues, where a higher growth rate was observed in the medium enriched with coconut fiber and lower in those containing bagasse. When studying these intrinsic factors, it was observed that an inadequate carbon‐nitrogen (C/N) ratio affected mycelial growth. This method of obtaining biomaterials is a promising alternative to lignocellulosic substrates as substrates for fungi with delignification capacity.