Background: Goniotomy-assisted transluminal trabeculotomy (GATT) is a minimally invasive technique that avoids conjunctival incision and is guided by light through the Schlemm’s canal using a microcatheter with an illuminated tip. This technique decreases intraocular pressure (IOP) by improving flow through the Schlemm’s canal. We present two cases of glaucoma patients who underwent GATT surgery for IOP control. Cases presentation: The first case is a 19-year patient with juvenile glaucoma that underwent GATT because of uncontrolled IOP with a successful outcome. The second case is a 64-year female patient with primary open-angle glaucoma who underwent GATT surgery because of uncontrolled IOP who presented a cyclodialysis secondary to the procedure, with an adequate IOP after surgery. Ultra biomicroscopy (UBM) was used to assess the anatomical changes associated with surgery. Conclusions: Goniotomy-assisted transluminal trabeculotomy is a safe technique but not free of risks and potential complications. Ultra biomicroscopy is a diagnostic aid that allows us to provide valuable information to evaluate the pre-surgical, post-surgical anatomy and possible complications to follow-up and guide the management in required cases.