Invasive prenatal testing (IPT) by amniocentesis (AMN) and chorionic villus sampling (CVS) are still relative common procedures in centralised fetal diagnostic centres. Beyond safety and efficacy performance accomplished issues, pain perception is a usual concern for patients, may affect the final decision process. The aim of this study was to identify in our population if the number of annual IPTs per operator is associated to patients' pain perception. In patients scheduled for IPT at our referral centre we prospectively determined the immediate pos procedure pain perception through an analogue pain perception scale 1 to 10. We analysed the association between reported pain perception and the annual number of procedures practiced by each of five operators. Statistical analysis was performed using Kruskal-Wallis and Mann–Whitney U test. A p-value of < 0.05 was considered statistically significant. 431 cases of IPT were practiced between 2018 and 2020. All procedures were performed by a close group of 5 operators. The pregnancy loss rate was 0.5 %. Patients perception of pain was statistically significantly higher when the operator realised less than 19 annual procedures and statistically significantly lower when the operator realised more than 52 annual procedures. Based on our results a minimal annual number of procedures per operator may warrant a low patients pain perception score and we propose it as a new frontier for quality assessment.