Nowadays, it is important to search for new alternatives that allow to find added value of microalgae biomass and thus, maximize economic scenarios of the biological refineries.The study of the residual biomass as a product of the previous lipid separation is interesting to take advantage of remaining carbohydrates, hydrolyzing them.The aims of this study were to define conditions of enzymatic hydrolysis of the carbohydrates included in the residual biomass of Chlorella vulgaris after lipid extraction.Firstly, the microalgae were grown, secondly, the lipids were extracted from the harvested biomass and finally, the residual biomass was pretreated through chemical hydrolysis.Considering the results of the acid pretreatment and the structural aspects of the microalgae, we used the chemical and enzymatic hydrolysis.The glucose, xylose, arabinose and hydrolysates were measured by using chromatography.The final outcomes were between 86.8 and 151.8 mg of reducing sugars (RS)/g of residual biomass on dry basis (brs).The previous chemical hydrolysis reached values between 11.1%-43.5% and the enzymatic between 34.4%-40.2%,together they achieved global values of up to 81% of saccharification.The high concentration of acid and the high enzyme/substrate ratio, increased the dose of pectinase, leading to higher yields of sugars released, with values above 140 mg/g brs.The enzymatic hydrolysis was enhanced with chemical pretreatment, being its contribution on average 58% of global saccharification.The outcomes were comparable with reports indicating a positive effect of pectinases on the degradation of Chlorella vulgaris polysaccharides using pretreatment, with saccharification of up to 80%.