Background: Amelogenesis imperfecta (AI) is a hereditary condition that affects the structure of tooth enamel and causes sensitivity, predisposition to cavities, and psychological problems. In Colombia, its frequency, magnitude, distribution, and behavior are unknown, so it is necessary to carry out prevalence studies to implement preventive actions. Purpose: To determine the prevalence of AI in patients who have attended the Pontificia Universidad Javeriana clinics in Bogotá. Methods: A retrospective cross-sectional observational study was carried out, whose sample included 1,394 medical records of patients who attended between January 2015 and December 2017. Results: The prevalence of AI was 0.6 %, corresponding to 8 people affected, 4 men and 4 women between the ages of 9 and 10 years. The most frequent phenotype was hypoplastic in 7 patients (87.5 %) and one person had a hypocalcified phenotype (12.5 %). Taurodontism was the most frequent anomaly in the 8 patients (100 %). Seven of the eight patients (87.5 %) had a family history of AI. All the individuals had a lower-middle socioeconomic level and came from urban areas. Conclusions: This study is the first approximation to determine the prevalence of AI in a group of the Colombian population. Although the prevalence was low, it is comparable with the findings of other studies.