The environmental isotopes are a tool for interpreting the hydrological processes related to underground environments. These are used for the validation of hydrogeological models, in terms of recharge, transit and discharge zones, as well as the identification of groundwater interactions with other water bodies and groundwater residence time in the hydrogeological units. The determination of the recharge processes have usually been validated from knowledge about the stable isotopes present in the water molecule (18O and 2H). At the regional level, this evaluation has been addressed through the construction of isoscapes combined with an understanding of the hydrological characteristics present in the study area and knowledge of conceptual hydrogeological models. The objective of this study is to apply a scheme for the analysis of potential groundwater recharge zones, from 18O and 2H spatial variability in precipitation, baseflow and groundwater, using spatial modelling tools. Based on a preliminary work done for the Antioquia department, a strategic area was chosen in which the results are more forceful and have greater implications in groundwater management: the Aburrá Valley-Colombia.