Objectives: Breast cancer is the most common cancer diagnosed in women, and microcalcification (MCC) clusters act as an early indicator. Thus, the detection of MCCs plays an important role in diagnosing breast cancer.Methods: This paper presents a methodology for mammogram preprocessing and MCC detection. The preprocessing method employs automatic artefact deletion and pectoral muscle removal based on region-growing segmentation and polynomial contour fitting. The MCC detection method uses a convolutional neural network for region-of-interest (ROI) classification, along with morphological operations and wavelet reconstruction to reduce false positives (FPs).Results: The methodology was evaluated using the mini-MIAS and UTP datasets in terms of segmentation accuracy in the preprocessing phase, as well as sensitivity and the mean FP rate per image in the MCC detection phase. With the mini-MIAS dataset, the proposed methods achieved accuracy scores of 99% for breast segmentation and 95% for pectoral segmentation, a sensitivity score of 82% for MCC detection, and an FP rate per image of 3.27. With the UTP dataset, the methods achieved accuracy scores of 97% for breast segmentation and 91% for pectoral segmentation, a sensitivity score of 78% for MCC detection, and an FP rate per image of 0.74.Conclusions: The proposed preprocessing method outperformed the state-of-the-art methods for breast segmentation and achieved relatively good results for pectoral muscle removal. Furthermore, the MCC detection module achieved the highest test accuracy in identifying potential ROIs with MCCs compared to other methods.