Abstract Most cytokines are present at reduced amounts in body fluids due to their biological features of production, release, and action mechanisms. The required time between sampling and their measurement is critical for diagnosis and treatment. Electrochemical nanobiosensors offer the possibility to be tailor‐made and cost affordable, producing direct and rapid readouts with low sample volume, explaining their feasibility in timely measurements and potential in designing unique and multiplexed Point‐Of‐Care (POC) testing platforms. This review summarizes and discusses the measurement limitations of the standard methods and the recent progress on electrochemical nanobiosensors as a plausible alternative to measuring them.