A prospective and experimental assessment of vitamin A supplementation effect on the levels of cytokines, total cholesterol (CT), LDL cholesterol, triglycerides (TG) and glucose (G) in 59 healthy adult subjects was performed. A single vitamin A capsule per week (retinol palmitate: 15.152 retinol equivalents [ER]/capsule) was supplied to each individual during one month (4 capsules in total). Cytokines interleukin 2, 4, 6, 10, TNF‐ α , IFN‐ γ , CT, HDL, LDL, TG and G were evaluated in blood at 0 day (before supplementation) and 28 day (after supplementation). The subjects registered by themselves the food intake every day during the following (28 days). At 28 day no change was observed in cytokines levels, suggesting that cytokines are not affected by vitamin A supplementation. An increase in CT, LDL y TG: 3,39%; 6,78% y 6,78 % respectively was observed (p<0,005), the diet had no effect on this increase. In addition, there was a reduction of 10,17% in HDL levels. As a conclusion, vitamin A supplement had a positive effect on CT, LDL, TG and G levels.