Resistant starch (RS) is that fraction of total ingested starch and its degradation products that escape from endogenous enzymatic action in the digestive tract and may contribute to the increase of beneficial bacteria in the cecum, thus contributing to improve the health status of the individual. The experiment involved 260 broilers randomly assigned to one of 4 treatments: control (C), mannan‐oligosaccharides (MOS), retrograded resistant starch from the S. Tuberosum potato (RST), and from the S. Phureja (RSP). Chickens were allocated in metabolism crates for digestibility study. Five birds from each dietary treatment were randomly selected at the end of the digestibility study for morphology measurements. Intestinal tissues were collected from the mid section of duodenum, jejunum and ileum to measure crypt depth and villus height. Data were analysis using complete randomized design. Average daily gain and feed intake were similar in all the groups (P>0.05). Birds fed RST and RSP had greater villus:crypt ratio in jejunum compared to C group (P<0.01) (10.48um and 10.08um vs 7.81um). In duodenum the greatest villus:crypt ratio was shown by the birds in the RST group (12.34um) compared to MOS (8.25um). The results suggest that potato resistant starch is a potential prebiotic.