Abstract The study of the phytochemical composition of seed oils is of upmost importance for the food and cosmetic industries, mainly considering their associated biological properties. Extraction of seed oils using supercritical fluids (SFE) is an ecological and green alternative to conventional extraction processes since it is able to provide with potent bioactive extracts, avoiding degradation and transformation of the compounds present originally in the raw material. The objective of the present work was the extraction of pure fractions of polar lipids and their chemical characterization using chromatographic techniques such as GC-MS and LC-DAD-MS/MS of blackberry ( Rubus glaucus ) and passion fruit ( Passiflora edulis ) seed oils extracted by supercritical carbon dioxide. Oleamides derived from oleic acid were identified as the main compounds in both samples; in particular, 9-octadecenamide was the major identified oleamide. Besides, the extract obtained from passion fruit showed to be a source of linoleic acid, while the SFE extract from blackberry presented important concentrations of vanillin. The chemical composition of these seed oils can be of high interest for their further use in cosmetics and food industry.