Use of biotechnological potential of native microorganisms as bio-inputs is having a great impact on agricultural systems. Plant Growth Promoting Rhizobacteria (PGPR), in addition to their beneficial effect on plant growth and on the availability of soil elements, also have an antagonistic effect against different pathogens. In this study, growth promotion mechanisms with emphasis on the antagonism of PGPR isolated from sugarcane and tomato crops were evaluated. Antagonism against Fusarium oxysporum f.sp lycopersici (Fol) was determined by dual tests, inhibition of germination and production of chitinases and endoglucanases. 52 isolates were evaluated and according to their results in dual tests 10 were selected for further analysis. Isolate GIBI127 showed the best percentage of Inhibition Germination (IG) of Fol (59.29%). Then, a selection index was calculated using results from gi, dual tests and growth promotion mechanisms to select five best isolates. Finally, these bacteria were evaluated for chitinases and endoglucanases production using Miller´s method. As a result, strain GIBI419 (Burkholderia cepacia) showed a higher production of these enzymes. Selected isolates have antagonistic potential along with plant growth promotion characteristics, which can be used for the development of microbial inoculants which allow the establishment of agricultural systems for tomato cultivation that are sustainable, efficient, and environmentally friendly.