We report the predation on the gecko Hemidactylus angulatus by the spider Ancylometes bogotensis, in the Tropical Dry Forest life zone located at Northern Tolima (Colombia). This short note is a contribution to the knowledge about the natural history of the referenced species because of the potential of A. bogotensis as a biological control agent of geckos that decreases its invasive potential. This is important due to the possibility to make populations of the gecko decline and to restrict the entry of these saurians to natural forested areas.