Salmonella spp. is classified as a Gram-negative bacterium and is characterized as a facultative anaerobe. The worldwide prevalence of Salmonella enteritidis is approximately 100 million cases. Aim: To study the effect of mananooligosaccharides and β-glucans (1,3) (1,6) for stimulating cellular immune response at the gastrointestinal level in the cecum of chickens to reduce Salmonella spp. colonization. Methods: A systematic review and meta-analysis were performed, including studies performed between 1980 and 2020. A total of 16 research papers with 28 treatments were chosen, and the statistical software Stat Graphics Stratus was used. Commands comparing the test with multiple ranges were used; groups treated with β-glucans (1,3) (1,6) in the cecum were used for determining the prevalence of Salmonella enteritidis, and groups not treated with β-glucans were used as control. The significance (P > 0.05) was validated by Tukey’s test and the corresponding linear regression equation was obtained. Results: There were differences between the prevalence of cecum-contaminated chickens in the β-glucan (1,3) (1,6) treated group and the control group. A mean of 29.60 ± 4.84 SD was observed in the treated group vs. 62.52 ± 47.6 SD in the control group (P = 0.0000), based on which it can be concluded that there is a highly significant difference in favor of the group treated with β-glucans (1,3) (1,6). A statistical contrast test was performed over the differences, which yielded a result in favor of using β-glucans (1,3) (1,6) because they decrease Salmonella spp. contamination of the cecum by 32.93 ± 13.3%. Conclusions: βGlucans (1,3) (1,6) can be used to reduce Salmonella spp. colonization in the cecum of chickens, which would consequently decrease the systemic transfer of this bacterium to other organs, especially to eggs that are for human consumption.