Diatomite is a powdering mineral mainly composed of diatom microfossils present in marine and lacustrine soils, which influences their physical and mechanical properties. Although many articles have been found in the literature concerning the influence of diatomite in the overall behavior of natural soils, few research efforts have been carried out to evaluate the influence of the diatom microfossil species on their shear resistance. Therefore, in this research, the influence of the diatomite species and the content in the peak and the residual shear strength of diatomite-fine grained soil mixtures was analyzed using the annular shear strength test. Scanning electron microscopy (SEM) and Atterberg limits were also carried out as additional tests to explain the interlocking effect between the microfossils and the soil. Overall, both diatomite species increased both peak and residual shear strength of the soil similar to dense sands. Nevertheless, the Mexican species reveal higher friction angle values compared with Colombian species.