The objective of the work was to evaluate the efficiency of Moringa oleifera seeds as a natural coagulant-flocculant in the treatment of water for human consumption. Seeds were collected from trees present in forest remnants of the city of Ibagué (Colombia), dried, pulverized, and subjected to the process of extraction of the active agents from organic solvents; samples were taken from river surface waters and with a Latin square design (DCL) that grouped two factors (water pH and extract dosage). The coagulant action was evaluated in the tests carried out with jar tests. According to the ranges of pH (4, 5, 6 and 7) and dosages (5, 10, 15 and 20 mL) used, a greater reduction of the turbidity values was obtained in the conditions of pH 6 with a dosage of 10 mL of coagulant solution, and pH 7 with a dosage of 10 mL of coagulant solution. The tests carried out showed that the coagulation-flocculation levels of moringa seeds do not vary significantly concerning the reports in the literature for Colombia. Their performance is associated with the factors established in the experimental design and maintains experimental efficiency against coagulants conventional used for the purification of water.