This research is related to the development of a customized Virtual Learning Environment that is able to identify learning styles of users, with the use of the Pedagogical Agent Doris.The objective is to identify the relation established among the Learning Styles, content presentation and interaction with Doris.The device Eye Tracker and the software Face Reader were used as methodological tools, so that the results of the user's interaction with the Virtual Environment were evaluated.This approach made it possible to observe that the preference related to the content presentation model is closely related to the orientation on the subject, being possible a variation according to the worked knowledge area.