La percepcion del riesgo es un factor importante asociado al error humano, e involucrado en la ocurrencia de accidentes laborales. Objetivo: Explicar la relacion que existe entre la percepcion del riesgo biologico y la accidentalidad laboral de los empleados de un hospital de Antioquia, en el ano 2019. Metodologia: Estudio explicativo transversal que empleo la metodologia EPRO (Evaluacion de Percepcion de Riesgo Ocupacional) y el software RISKPERCEP a traves de una encuesta estructurada a todos los trabajadores (N=50) de la institucion. La informacion estadistica se analizo con el software SPSSv24. Resultados: Se encuentra un perfil de subestimacion del riesgo biologico laboral en las diferentes areas de la institucion. A la accidentalidad laboral se asocian factores como el tiempo desarrollando la misma actividad y la percepcion del estado de salud. Conclusion: Los trabajadores con mayor experiencia y exposicion al riesgo biologico laboral subestiman el riesgo. The perception of risk is associated with the occurrence of occupational accidents. For health companies it is fundamental to know this phenomenon, thus favoring the implementation of coping and prevention strategies, which contribute to the reduction of work accidentality. Objective: To analyze the perception of biological risk and the associated factors with occupational accidents through the ORPE (Occupational Risk Perception Evaluation) method and the RISKPERCEP software of the employees of a hospital in Antioquia, Colombia, in 2019. Methodology: Cross-sectional explanatory study that used the ORPE methodology and the RISKPERCEP software through a structured survey of all the workers (N=50) of the institution. Statistical information was analyzed with SPSSv24 software. Results: An underestimation profile of occupational biological risk is found in the different areas of the institution. Factors such as time developing the same activity and perception of the state of health are associated with work accidentality. Conclusion: Workers with more experience and exposure to occupational biological risk underestimate the risk.