In Colombia, the genus is currently represented by a single species A. canalis recorded by Galvis & Moreno [2016] from both sexes from Sapzurro in the Chocó department, on the border with Panama.During the ecological and biodiversity study of spider communities of the Caribbean mangrove forests in the department of Bolívar of Colombia, we have collected males and females of an unknown Anasaitis species.This new species, which is described hereinafter, is the first true South American representative of the genus. Material and methodsThe material studied is deposited in the Arachnological Collection of Instituto de Ciencias Naturales of the Universidad Nacional de Colombia (ICN-Ar, Eduardo Flórez), Bogotá, Colombia.Digital photographs were taken with a Leica MC-170 HD digital camera attached to a Leica M205A stereomicroscope, and then combined by using the image stacking software Leica Application Suite version 4.6.0.The map was prepared in the Geographic Information System QGIS "A Coruña" (version 3.1, under license CC BY-SA 3.0).Raster files from NaturalEarth© and DivaGis© were used to perform the distributional map of the recordsThe format of description follows Galvis [2015].Abbreviations used in the text and figures are as follows: a.s.l.above sea level; CO -copulatory opening; d -dorsal; E -embolus; FD -fertilization ducts; ID -insemination ducts; m -meters; me -medial; OC -ocular quadrangle; p -prolateral; r -retrolateral; RTA -retrolateral tibial apophysis; S -spermatheca; Spe -spermophore; TLproximal tegular lobe; v -ventral