The food-energy-water (FEW) nexus approach has emerged as an alternative for managing these resources more efficiently. Work from studies conducted in the FEW nexus in Latin America is scarce in the scholarly literature. This study aims to develop a framework for water management at the FEW Nexus, with a focus on Colombia. The study focuses on a typical mixed land-use watershed in the Andean region with specific objectives being to: (1) characterize the watershed with respect to land use, climate, water resources, and other factors pertinent to the nexus; (2) explore the relationship between factors in the FEW nexus that may affect water management in terms of quality and availability; and (3) propose a methodology for conducting a FEW Nexus analysis for watersheds located in the Andean region. The results indicate that the Pereira/Dosquebradas urban area has a significant impact on the FEW nexus components in the Otun River Watershed (ORW). Subsequently, an urban FEW nexus framework is proposed for its implementation at the watershed.