The risk of transmission of microorganisms in aesthetic and beauty centers is high when mitigation measures are not adopted; thus, it is necessary to constantly review the conditions of these centers, in order to prevent diseases and make the corresponding decisions.To assess biosafety conditions of beauty centers in Valledupar, Colombia.This study followed a descriptive methodology and was based on the administration of a survey focused on determining which services are provided in beauty centers, on which activities are developed by their staff, and the conditions under which services are provided.Study results that 93% of centers are legally constituted; furthermore, the most commonly provided service was hairdressing, with 21%. Only 9% of workers were covered by a social insurance system. In terms of social insurances, centers did not comply with minimum requirements.The aesthetic centers in Valledupar are not complying with requirements from biosafety protocols set forth by legislation and are thus adopting inadequate procedures.