Hydroptilidae is one of the most diverse families of the order Trichoptera in the world. Neotropical fauna has 36 genera. In Colombia, 14 have been reported in the larval stage and 11 in the adultstage. In the present study, a Byrsopteryx larva is registered for the first time in Colombia. The larvawas found in the Orotoy River (919 MASL), a stream from the foothills of the eastern flank of the country’s Eastern Cordillera (Orinoquia) characterized as waterfall current with riffle on rocks and pebbles, preserved riparian vegetation with open canopy, and clay-loam soil. The water temperature was 19.7 ºC, pH 5.8, conductivity 15.6 uS/cm, and dissolved oxygen 9.1-9.7 mg/lO2. Given the high diversity of aquatic ecosystems and the limited knowledge of Hydroptilidae genera in Colombia, we hope to open the way for new research and discovery of more species in the country.