Objective: To determine the characteristics of power, speed, heart rate, kinematics, stabilometric and electromyographic pedaling on a road bike in a professional and amateur cyclists. Methodology: The study type is a case study conducted with 2 cyclists (one professional and one amateur) who are tested for kinematics, stabilometry and electromyography with Bioengineering technology. Results: The power shows linear tendency being greater in the professional cyclist compared to the amateur. The speed with linear behavior is greater in the professional cyclist. The kinematic analysis shows greater consistency in the movements performed by the professional cyclist. The differences are more evident in the trunk and ankle, extreme-proximal and distal joints of the kinetic chain. Muscle activity showed more stable patterns and larger amplitudes in the professional cyclist, according to stability, the Romberg index in both cyclists is normal with a value of 0,879 for the professional and 0,704 for the amateur. Conclusion: The resistance is related to the level of training of the athlete, indicating that the greater the training, the less compensation with other body segments; optimizing the sporting gesture and performance. In relation to static and dynamic stability, there are no relevant differences between the participants.